Structure-based analysis of these mutations provides an insight into the alternating access mechanism of GLUT1 and other members of the sugar porter subfamily. This structure allows accurate mapping and potential mechanistic interpretation of disease-associated mutations in GLUT1.

The full-length protein, which has a canonical major facilitator superfamily fold, is captured in an inward-open conformation.

Here we report the crystal structure of human GLUT1 at 3.2 Å resolution.

Despite decades of investigation, the structure of GLUT1 remains unknown. Dysfunctional mutations may lead to GLUT1 deficiency syndrome, whereas overexpression of GLUT1 is a prognostic indicator for cancer. The glucose transporter GLUT1 catalyses facilitative diffusion of glucose into erythrocytes and is responsible for glucose supply to the brain and other organs. Diversity, Equity, Inclusion, and Access.Citation, Usage, Privacy Policies, Logo.Biologically Interesting Molecule Reference Dictionary (BIRD).Combined with its well flexible and biocompatible features, it holds considerable prospects in the development of wireless wearable biosensors for continuous blood glucose monitoring. Finally, tests on healthy volunteers were conducted to further validate the feasibility of this as-proposed system. Highly sensitive and accurate detection of ISF glucose could be achieved with an LOD of 0.26 mM over a 0–15 mM range. Additionally, a self-made diffuse cell and an ex vivo model were developed to demonstrate the efficacy of ISF extraction based on reverse iontophoresis technology. A three-dimensional graphene aerogel combined with Prussian blue was introduced as an electron mediator, providing suitable support for glucose oxidase (GO x) immobilization, highly boosting the detection sensitivity. Herein, we present a screen-printed iontophoretic biosensing system to extract ISF noninvasively and perform in situ instant glucose detection. Interstitial fluid (ISF) glucose, displaying an excellent correlation with blood glucose, is highly desired to address the limitations of invasive and minimally invasive glucose detection.

Diabetes and impaired glucose regulation (IGR) threaten the lives and health of numerous patients.

Glucose structure download